人類胚胎干細胞研究是當前最熱門的科研課題之一,經由無限制的增殖及適當的誘導分化后,胚胎細胞干細胞可分化成人體中各種類型的細胞,如神經細胞、心肌細胞及軟骨細胞,這些特點造就胚胎干細胞未來在人類醫學健康應用上不可估量的前景。
傳統干細胞培養過程中必需添加的異種動物(非人類)成分,如牛血清或豬血清,使之無法完全排除人畜共染病毒、朊病毒(prion)及支原體污染細胞的可能性,這是造成人類干細胞進入臨床應用的主要障礙之一。經由使用成分明確、全合成的無血清干細胞培養基可有效避免人畜共染疾病的問題,同時全合成培養基無批間差異,培養條件容易保持一致,實驗的重復性大為提高。應用無血清干細胞培養基可為干細胞在心血管疾病、神經性退化及癌癥的臨床治療方面打開了一扇大門。
為了滿足科研與生物醫藥企業的需求,bi公司與以色列國家科學研究院(technion-israel institute of technology)的dr. itskovitz共同合作開發了最新一代、擁有自主知識產權的人類胚胎干細胞(hesc)和誘導胚胎干細胞(ipsc)專用無血清完全培養基——nutristem?。
dr. joseph itskovitz己在stem cells, stem cell dev, pnas, nature及science等雜志發表近百篇文章,并有三項胚胎干細胞培養與分化誘導國際專利,同時也是1998年第一篇分離人類胚胎干細胞論文的共同作者,現任職于以色列國家科學研究院rambam medical center。美國國家衛生研究院干細胞庫中批準用于科研的22株人類胚胎干細胞由其團隊提供了8株。
embryonic stem cell lines derived from human blastocysts. j. a. thomson, j. itskovitz-eldor, et. al., science. vol. 282, no. 5391, 1145-1147. 1998.
nutristem
? hesc xf, xeno-free medium for human es & ips cell culture, with hsa
貨號:05-100-1a/b,人類胚胎干細胞無血清培養基(含人血清白蛋白)
開瓶即可使用的完全培養基,不含異源動物成分,添加了醫療級的人血清白蛋白(hsa:human serum albumin),以適用于無滋養層培養法(feeder-free),如matrigel?或conditioned medium培養。
af nutristem? hesc xf, xeno-free medium for human es & ips cell culture, without hsa
貨號: 05-102-1a/b,人類胚胎干細胞無血清培養基(不含人血清白蛋白)
開瓶即可使用的完全培養基,不含任何動物來源成分,適用于鼠胚胎纖維原細胞(mouse embryonic fibroblasts-mef)及人包皮纖維原細胞(human foreskin fibroblasts-hff)滋養層培養法。
nutristem?已發表參考文獻:
1. human and mouse adipose-derived cells support feeder-independent induction of pluripotent stem cells. sugii et al. pnas. vol. 107, no 8, 3558-3563, 2009.
2. current technology for the derivation of pluripotent stem cell lines from human embryos. hasegawa et al., cell stem cell. vol. 6, 521-531, 2010.
3. progress and challenges in optimiztion of human pluripotent stem cell culture. ge et al., current stem cell research & therapy. vol. 5, 207-214, 2010.
4. highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mrna. warren et al., cell stem cell. vol. 7, 1-13, 2010.
5. fibrin microbeads (fmb) loaded with mesenchymal cells support their long term survival while sealed at room temperature. gorodetsky et al. tissue engineering part c: methods. online ahead of editing, march 2011.
6. site-specific gene correction of a point mutation in human ips cells derived from an adult patient with sickle cell disease. zou, et al. blood. august 2011.
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