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style="width:25%;color:#333;text-align:center;font-size:.7rem;border-bottom:1px solid #ececec;border-right:1px solid #ececec;" valign="top">數量</td> <td style="width:75%;color:#666;font-size:.7rem;border-bottom:1px solid #ececec;">大量</td> </tr> <tr> <td style="width:25%;color:#333;text-align:center;font-size:.7rem;border-bottom:1px solid #ececec;border-right:1px solid #ececec;" valign="top">供應商</td> <td style="width:75%;color:#666;font-size:.7rem;border-bottom:1px solid #ececec;">康朗生物</td> </tr> <tr> <td style="width:25%;color:#333;text-align:center;font-size:.7rem;border-bottom:1px solid #ececec;border-right:1px solid #ececec;" valign="top">檢測限</td> <td style="width:75%;color:#666;font-size:.7rem;border-bottom:1px solid #ececec;">見說明書</td> </tr> <tr> <td style="width:25%;color:#333;text-align:center;font-size:.7rem;border-bottom:1px solid #ececec;border-right:1px solid #ececec;" valign="top">檢測方法</td> <td style="width:75%;color:#666;font-size:.7rem;border-bottom:1px solid #ececec;">酶聯免疫</td> </tr> <tr> <td style="width:25%;color:#333;text-align:center;font-size:.7rem;border-bottom:1px solid #ececec;border-right:1px solid #ececec;" valign="top">應用</td> <td style="width:75%;color:#666;font-size:.7rem;border-bottom:1px solid #ececec;">科研</td> </tr> <tr> <td style="width:25%;color:#333;text-align:center;font-size:.7rem;border-bottom:1px solid #ececec;border-right:1px solid #ececec;" valign="top">標記物</td> <td style="width:75%;color:#666;font-size:.7rem;border-bottom:1px solid #ececec;">人</td> </tr> <tr> <td style="width:25%;color:#333;text-align:center;font-size:.7rem;border-bottom:1px solid #ececec;border-right:1px solid #ececec;" valign="top">樣本</td> <td style="width:75%;color:#666;font-size:.7rem;border-bottom:1px solid #ececec;">血清/組織/尿液</td> </tr> <tr> <td style="width:25%;color:#333;text-align:center;font-size:.7rem;border-bottom:1px solid #ececec;border-right:1px solid #ececec;" valign="top">規格</td> <td style="width:75%;color:#666;font-size:.7rem;border-bottom:1px solid #ececec;">48T/96T</td> </tr> </table> <div style="padding:0.4rem;"> <div class="clearfix">  <br><p>人白介素3(il-3)elisa試劑盒</p>的原理 elisa 的基礎是抗原或抗體的固相化及抗原或抗體的酶標記。結合在固相載體表面的抗原或抗體仍保持其免疫學活性，酶標記的抗原或抗體既保留其免疫學活性，又保留酶的活性。<p>人白介素3(il-3)elisa試劑盒</p>在測定時，受檢標本（測定其中的抗體或抗原）與固相載體表面的抗原或抗體起反應。用洗滌的方法使固相載體上形成的抗原抗體復合物與液體中的其他物質分開。再加入酶標記的抗原或抗。<br><br> <imgx src="http://imgx.dxycdn.com/trademd/upload/userfiles/imagex/2015/08/a1438846615_small.jpg" stylex="width: 388.0px;height: 168.0px;"><br><br>a.分子量大。合成肽采用化學方法制備，由于工藝的局限，合成數量有限，只能達到數百個氨基酸;而利用基因工程制備的抗原，分子量更大。<br>b.穩定性好。<p>人白介素3(il-3)elisa試劑盒</p>包被的抗原的穩定性可使試劑盒的效期得到保證，早期以合成肽為包被抗原的試劑盒效期只有3-4 個月，采用基因工程抗原后效期大大延長了。<br>c.基因工程抗原將特異性抗原決定簇基因融合表達，表達產物包含更多的抗原決定簇，可提高試劑盒的靈敏度，提高檢出率。<br>d.純化難度大?；蚬こ炭乖募兓夹g難度較大。<br>合成多肽抗原是根據蛋白質抗原分子的某一抗原決定簇的氨基酸序列人工合成的多肽片段。合成肽抗原有以下特點： a.分子量太小;b.一般只含有一個抗原決定簇;c.純度高;d.穩定性差。<br><br><p>人白介素3(il-3)elisa試劑盒</p>棋盤試驗的操作方法:<br>(1)將抗原按一定的梯度倍比稀釋后，按照elisa從上到下(或者從左到右)的順序包被。<br>(2)將抗體按一定的梯度倍比稀釋后按照elisa從左到右或者從上到下加入。<br>(3)二抗的稀釋倍數是一定的，然后顯色，讀值。<br>od值的測定時，波長要根據顯色劑的不同而定，一般上大家都使用tmb顯色，450nm讀值。根據讀值得結果可以選定合適的抗原和抗體效價，<p>人白介素3(il-3)elisa試劑盒</p>這就是棋盤實驗的目的，如果抗原包被量已知而二抗的工作濃度不確定的話就用一抗和二抗作棋盤試驗。<br><br> </div> </div> </li> </ul> </div> </div>  <div class="kj_footer"> <div class="footer_inner"> <div style="line-height:1.6rem;background:#fff;"> <a href="http://www.faihu.com/chanpin/" target="_self" class="external"> 產品 </a> <a href="http://www.faihu.com/changjia/" target="_self" class="external"> 廠家 </a> <a href="http://www.faihu.com/cas/" target="_self" class="external"> 化工詞典 </a> <a href="http://www.faihu.com/structure/" target="_self" class="external"> 結構式搜索 </a> <a href="http://www.faihu.com/msds/" target="_self" class="external"> MSDS </a> <a href="http://www.faihu.com/news/" target="_self" class="external"> 資訊 </a> <a 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